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jangles ago

http://www.sigmaaldrich.com/catalog/product/sigma/ckozfn16370?lang=en&region=US PAX2 - market price Just $5k for 10 aliquots. The pedos are just a tool for them to increase peptide production.

Apothecarist ago

It's been a few years since I've stepped foot in a lab but that link is for a Zinc Finger Nuclease. These enzymes are used to create "knockout" strains for research. The one you linked is to specifically "knockout" the human PAX2 gene. If you are not familiar with this technology, knockouts are used to study the effects of removing specific genes from the genome. So this one in particular would be used to study the effects of a missing PAX2 gene (and consequently, the importance of a PAX2 gene). PAX2 is involved in many signaling pathways and has been studied for a long time (I learned about it in many of my undergraduate courses).

https://www.researchgate.net/publication/233383105_Signaling_pathways_of_PAX2_and_its_role_in_renal_interstitial_fibrosis_and_glomerulosclerosis (not the best image but I don't feel like looking any further atm)

As a side note, nothing is cheap in the biotech industry. It is not uncommon for a peptide or enzyme to cost hundred/thousands of dollars, even if you are only buying a few microliters. With that said, research is typically done on animal models. But I'm sure there's an interest in doing research on human models...and obtaining certain enzymes/peptides/tissues.

Apothecarist ago

It is true that the HeLa cell line is the main cell line used for a lot of research today (I've worked with it), and it is because they do not die like other cell lines. Regular cells cannot thrive in the lab without the rest of the body to support it. The HeLa cell line is cancerous - it was taken from Henrietta Lack's biopsied tumor (These cell lines are cancerous because of HPV - I'm not sure what they mean when they say they took it without her permission...but HPV is what they test for in a pap smear. Perhaps she gave them a sample, they noticed it didn't die like the others and they saved it/replicated it without her consent?). These cells replicate in nutrient agar very well, while previous cell lines died after a few days (imagine how much this stunted research).

Ironically, when I graduated from school my lab instructor gave me the book "The Immortal Life of Henrietta Lacks by Rebecca Skloot" and I have yet to read it (maybe I will now). Looking it up on wikipedia, there are a few things that strike me as odd:

  • HeLa cells were used by Jonas Salk to test the first polio vaccine in the 1950s.
  • In the spring of 1953, a cell culture factory was established at Tuskegee University to supply Salk and other labs with HeLa cells.

There is a lot of controversy surrounding both Salk/the polio vaccine, and especially Tuskegee University (Tuskegee syphilis experiment) "Just weeks after the Salk vaccine had been declared safe, more than 200 polio cases were traced to lots contaminated with virulent live polio strains manufactured by the Cutter Laboratories" http://www.history.com/news/8-things-you-may-not-know-about-jonas-salk-and-the-polio-vaccine

Interesting, Cutter Laboratories -- while doing this investigation I came across the news that Hillary/Bill were knowingly allowing the sale of HIV/Hep-C blood to hemophiliacs https://www.reddit.com/r/conspiracy/comments/4jv950/clinton_scandal_you_may_not_have_heard_of/
Cutter Laboratories was owned by Bayer. Bayer and Monsanto just formed a merger, and Hillary has been dubbed the “Bride of Frankenfood” for accepting donations and advocating GMOs on their behalf. Not to mention Bayer was responsible for the development and testing of Zyklon B on Jewish slave laborers during the Holocaust. They admitted it http://www.nydailynews.com/archives/news/bayer-nazi-role-article-1.701925. Bayer also invented chlorine gas and mustard gas. Monsanto helped develop the first nuclear bomb during the Manhattan project.

As a biochemist, for a few years now I have been interested in HIV and Ebola. There's a lot of controversy surrounding their seemingly "out of the blue" outbreaks. Both viruses/similar viruses were apparently being worked on in Africa, out of US funded labs. Their Simian counterparts have been studied as well.

  • For HIV: Look into the hepatitis B vaccine, its contamination (The polio vaccine was found to be contaminated with SV40). Rumor has it, the Hep B vaccine might’ve been contaminated with something like HIV. Look into Simian immunodeficiency viruses (we have done experiments with them…notice how the Rhesus factor comes into play here “Extensive studies in sooty mangabeys have established that SIVsmm infection does not cause any disease in these animals, despite high levels of circulating virus. However, if this virus infects an Asian or Indian rhesus macaque, the animal will develop simian AIDS (SAIDS)” – from Wikipedia. Sooo Rh+ = HIV, Rh- = no HIV – put Rhesus monkey into wikileaks, notice the US was dead set on obtaining Rhesus monkeys, and they were even starting a farm for research purposes. This could be for regular things such as the development of the RhoGAM vaccine, or hey who knows…) Look into Dr. Robert Gallo, he "discovered" HIV. He also inoculated monkeys with HIV and released them back into the wild. How about the now declassified U.S. Special Virus Program?

  • For Ebola: See George Soros, Kenema, USAMRIID, CDC patent CA2741523A1...why are Africans avoiding the Red Cross and claiming it is them that gave them all ebola? and now we are working on a vaccine? (It also doesn't take much to look into the companies developing it to see the fishy history/funding there...but with science comes a lot of mistakes, and it's expensive as we’ve established). Additionally, remember the period of time when Ebola broke out Boko Haram was apparently kidnapping a bunch of children? I could’ve sworn when I was doing research back then that I’d seen an article where the African people were saying it wasn’t true…

  • Now as for a weird connection, how about that doctor that claims to have cured Ebola victims using HIV drugs? Why was he reprimanded for doing so? What’s the molecular link between HIV and Ebola (this is what fascinates me)? http://www.cnn.com/2014/09/27/health/ebola-hiv-drug/

I’ve done this research multiple times over the past few years so a lot of what I am saying is coming off the top of my head. Also the sources I'm googling atm are not that credible and I have to go in a minute so I will not cite any sources but if you dig into this rabbit hole...it gets weird. I will try to delve into it and link some sources over the next few days.

Modern science has made tons and tons of progress since the 1950's. There are many ethical questions involved...animal research, stem cell research, genetic modification and the human genome project, etc. A lot of experiments that go well in animal models...do not go well in clinical trials with humans (and vice versa). Besides the question of ethics, experimenting on humans would be the next step. Take away ethics - add money, warfare, the possible quest for immortality into the mix. If people are willing to rape, murder, traffic and harvest organs from others - especially in an organized, underground matter, I cannot see why there wouldn't be a select few willing to do human experiments. Just knowing about this HeLa cell line, the Tuskegee experiments, MkUltra and the list goes on (https://en.wikipedia.org/wiki/Unethical_human_experimentation_in_the_United_States)) makes it incredibly plausible that we’re into some sketchy shit. While the satanic ritual abuse seems to happen, it’d seem a waste to me that they’d just dump perfectly good human tissue when they could be using it for stuff such as the aforementioned.

jangles ago

Hisv bengazzi comes to mind. Do you know much about that?

Apothecarist ago

https://en.wikipedia.org/wiki/HIV_trial_in_Libya

Is that what you are referring to? If so, no I hadn't heard about it until I googled it! Thanks for the info.

jangles ago

MSM does great journalism, that is why.